| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Lactobacillus acidophilus | GCGGAAACATCAAACCGTCC | TGCTGAGCCAATCGTCCTG | 60.11 | 60.08 | 218 | 142 |
100.00%
|
100.00%
|
| Primer 2 | Lactobacillus acidophilus | ACCACGCTGGCTTTACTGG | TCGATTGGGCAGTGGTCTG | 60.30 | 59.70 | 250 | 135 |
100.00%
|
50.00%
|
| Primer 3 | Lactobacillus acidophilus | TGGCGTTTTAAGCGTGGC | CATCATCCCCAGCAGTTTGC | 59.36 | 59.54 | 203 | 133 |
100.00%
|
50.00%
|
| Primer 4 | Lactobacillus acidophilus | ACCGGACGGAACTTGTCAG | ACAACTGGTGCAACTGGTTC | 59.63 | 58.90 | 246 | 133 |
100.00%
|
50.00%
|
| Primer 5 | Lactobacillus acidophilus | TCGCGATACATCCGGATTGG | ACCAAACTAGCTCCTGAGACC | 60.04 | 59.10 | 258 | 132 |
100.00%
|
100.00%
|
| Primer 6 | Lactobacillus acidophilus | GCAAACACCGGTTACAGTGC | GCTGGTGCAGGGATCACAT | 60.32 | 60.08 | 180 | 129 |
100.00%
|
100.00%
|
| Primer 7 | Lactobacillus acidophilus | GGCCACGTGCTACTTTCAAC | ACTCGCACACCAACTGCA | 59.76 | 59.81 | 271 | 128 |
100.00%
|
100.00%
|
| Primer 8 | Lactobacillus acidophilus | AATGGGAGAGCGGACAAGC | AGGCAAATCCGCAGATAGCA | 60.08 | 59.82 | 222 | 128 |
100.00%
|
50.00%
|
| Primer 9 | Lactobacillus acidophilus | AATGGGAGAGCGGACAAGC | GGCAAATCCGCAGATAGCA | 60.08 | 58.29 | 221 | 128 |
100.00%
|
50.00%
|
| Primer 10 | Lactobacillus acidophilus | AATGGGAGAGCGGACAAGC | GCTCCAGCAATTGCCCAAA | 60.08 | 59.33 | 196 | 127 |
100.00%
|
50.00%
|